Fig. 2

ZNF468 promotes the growth and migration of BC cells. A Immunoblotting analysis of ZNF468 in MCF-7 cells transfected with empty control and ZNF468 overexpression plasmids. GAPDH was used as internal control. B Cell viability was measured by CCK-8 assay for MCF-7 cells transfected with empty control and ZNF468 overexpression plasmids. *p < 0.05. **p < 0.01. C Colony formation assay was performed for Ctrl and ZNF468 overexpressing MCF-7 cells. **p < 0.01. D Immunoblotting detection of ZNF468 in MDA-MB-231 cells transfected with siCtrl, siZNF468#1 and siZNF468#2. GAPDH was used as internal control. E, F CCK-8 (E) and colony formation (F) assay was performed to analyze the cell proliferation of siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. *p < 0.05. **p < 0.01. G Cell apoptosis was checked by Annexin V/PI staining on flow cytometry system for siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. **p < 0.01. H Cell migration was checked by Transwell asasy for siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. **p < 0.01. For immunoblotting assays, the blots were cut prior to hybridisation with antibodies