Fig. 4

ZNF468 potentiates the expression of TFAM at transcriptional and protein level. A The positive genes correlated with ZNF468 in BC patients were analyzed based on TCGA database. B Spearman correlation between ZNF468 and TFAM was analyzed in BC patients from TCGA database. p < 0.001. C Analysis of TFAM transcript was performed in cancer (n = 1085) and normal tissues (n = 291) derived from BC patients. D, E The mRNA (D) and protein (E) level of TFAM was detected by RT-qPCR in Ctrl and ZNF468 overexpressing MCF-7 cells, and in siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. **p < 0.01. F Relative mtDNA copy number was measured by RT-qPCR assay in Ctrl and ZNF468 overexpressing MCF-7 cells, and in siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. *p < 0.05. G Dual luciferase activity of TFAM promoter was checked in Ctrl and ZNF468 overexpressing MCF-7 cells, and in siCtrl, siZNF468#1 and siZNF468#2 MDA-MB-231 cells. **p < 0.01. H ChIP-qPCR assay was performed to determine the binding of ZNF468 on the promoter of TFAM gene in MCF-7 cells. **p < 0.01. For immunoblotting assays, the blots were cut prior to hybridisation with antibodies