Fig. 1

AH induces GSDMD activation through the non-canonical inflammasomes pathway. Alcoholic hepatitis (AH) mouse model was induced by administering alcohol via gavage directly into the animal's stomach in normal mice. At the endpoint of each experiment, the blood and liver tissues were collected. A The injury of liver tissues showed by HE staining. B The serum levels of AST, ALT, TC and TG in control or AH mice were measured by commercial kits. C The serum levels of IL-1β and IL-18 in control or AH mice were measured by ELISA kits. D The expression of GSDMD-N, NLRP3, and Caspase 11 in liver tissues of control or AH mice were measured by IHC. Data are presented as mean ± SD (n = 6 mice per group). Unpaired t-test was performed for statistical analysis. **p < 0.01