Fig. 6

GSDMD mediates hepatocyte pyroptosis by regulating mitochondrial function in AH. GSDMD downregulation shRNA (sh-GSDMD) or Drp1 downregulation shRNA (sh-Drp1) or negative control shRNA (sh-NC) lentivirus were applied to AH mice by tail intravenous injection. At the endpoint of each experiment, the blood and liver tissues were collected. A The injury of liver tissues was showed by HE staining. B The serum levels of AST, ALT, TC and TG in control or AH mice were measured by commercial kits. C The serum levels of IL-1β and IL-18 in control or AH mice were measured by ELISA kits. D The protein expression of NLRP3, GSDMD-N, and Drp1 in liver tissues of control or AH mice was determined by western blot. E The gene expression of mtDNA (ND1 and ND2) in liver tissues of control or AH mice was determined by qPCR. F The level of ATP in liver tissues of control or AH mice were measured by ATP kits. Data are presented as mean ± SD (n = 6 mice per group). *p < 0.05, **p < 0.01. G The mitochondria structure of hepatocytes in liver tissues of control or AH mice were imaged by TEM.