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Figure 2 | Cell Division

Figure 2

From: Eg5 steps it up!

Figure 2

Schematic showing in vitro assay designs for Eg5 motor studies. (A) Depiction of a fluorescence-based assay used to demonstrate purified full length Eg5 tetramers are capable of crosslinking and sliding microtubules in vitro [13]. Unlabeled Eg5 motors bind to fluorescent, polarity-marked microtubules, causing the microtubules to slide apart. (B) Schematic showing optical trapping assay used to observe processive movement of Eg5 dimers [30]. His-tagged motors are attached to streptavidin-coated beads through a biotinlyated PentaHis antibody. Coverslip surfaces are precoated with poly-L-lysine-graft- poly(ethylene glycol) polymers to prevent surface-induced denaturation of Eg5 at the glass interface. Polymers are biotinylated to allow the specific attachment of biotinlyated microtubules via a streptavidin linkage.

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