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Table 1 Phosphorylated residues on Cdc25 and Wee1.

From: Cdc25 and Wee1: analogous opposites?

  Xenopus Site Human Site +/- Kinase Proposed function References
Cdc25 T48   + Cdc2, ERK Enhances activity and required for Cdc2 activation, binds to Pin1 22, 29, 48, 50, 51
  T67   + Cdc2, ? Required for Cdc2 activation, binds to Pin1 22, 48, 50, 51
  T138 T130 + Cdk2, ERK Promotes 14-3-3 release, may create binding site for Plk1 21, 29, 31, 32
   S198 + Plk1 Promotes nuclear translocation 41
  S205 S168 +/- ERK, p38/JNK Increases activity in Xenopus/Inhibits activity in mammalian cells 29, 54, 53
  S285 S214 + Cdc2 Promotes PP1-mediated dephosphorylation of S287 46–48
  S287 S216 - Chk1, Chk2, C-TAK, CamKII, PKA Binding site for 14-3-3, blocks nuclear import 5–20, 58
Wee1 S38   - Cdc2 Promotes degradation via Tome-1 83, 86
  T53   - Cdc2 Regulates protein stability 83
   S53 - Plk1 Promotes degradation via β-TrCP 87, 88
  Y90   + Wee1 Promotes activity 78
  Y103   + Wee1 Promotes activity 78
  T104   - Cdc2 Required for Wee1 inactivation 83
  Y110   + Wee1 Promotes activity 78
   S121 - CK2 Promotes degradation via β-TrCP 87
   S123 - Cdc2 Promotes Plk1 binding and degradation via β-TrCP 87, 88
  T150   - Cdc2, ? Required for Wee1 inactivation, binds top Pin1 83, 84
   Y295 + Wee1 Promotes activity 77
   Y362 + Wee1 Promotes activity 77
  S549 S642 +/- Chk1/Akt Promotes 14-3-3 binding and increases activity/Promotes cytoplasmic localization 58, 75–77
  1. The effect of phosphorylation at specific residues is listed as activating (+) or inactivating (-).