Cdc25 and Wee1: analogous opposites?

Cell Division

Table 1 Phosphorylated residues on Cdc25 and Wee1.

	Xenopus Site	Human Site	+/-	Kinase	Proposed function	References
Cdc25	T48		+	Cdc2, ERK	Enhances activity and required for Cdc2 activation, binds to Pin1	22, 29, 48, 50, 51
	T67		+	Cdc2, ?	Required for Cdc2 activation, binds to Pin1	22, 48, 50, 51
	T138	T130	+	Cdk2, ERK	Promotes 14-3-3 release, may create binding site for Plk1	21, 29, 31, 32
		S198	+	Plk1	Promotes nuclear translocation	41
	S205	S168	+/-	ERK, p38/JNK	Increases activity in Xenopus/Inhibits activity in mammalian cells	29, 54, 53
	S285	S214	+	Cdc2	Promotes PP1-mediated dephosphorylation of S287	46–48
	S287	S216	-	Chk1, Chk2, C-TAK, CamKII, PKA	Binding site for 14-3-3, blocks nuclear import	5–20, 58
Wee1	S38		-	Cdc2	Promotes degradation via Tome-1	83, 86
	T53		-	Cdc2	Regulates protein stability	83
		S53	-	Plk1	Promotes degradation via β-TrCP	87, 88
	Y90		+	Wee1	Promotes activity	78
	Y103		+	Wee1	Promotes activity	78
	T104		-	Cdc2	Required for Wee1 inactivation	83
	Y110		+	Wee1	Promotes activity	78
		S121	-	CK2	Promotes degradation via β-TrCP	87
		S123	-	Cdc2	Promotes Plk1 binding and degradation via β-TrCP	87, 88
	T150		-	Cdc2, ?	Required for Wee1 inactivation, binds top Pin1	83, 84
		Y295	+	Wee1	Promotes activity	77
		Y362	+	Wee1	Promotes activity	77
	S549	S642	+/-	Chk1/Akt	Promotes 14-3-3 binding and increases activity/Promotes cytoplasmic localization	58, 75–77

The effect of phosphorylation at specific residues is listed as activating (+) or inactivating (-).

ISSN: 1747-1028