Figure 1
From: Complement susceptibility in glutamine deprived breast cancer cells
Optimal NHS Dilution for Complement-mediated lysis of human breast cancer cell lines. The Bcap37 and MCF7 cells (1 × 105) were sensitized 20 μg rabbit antibody to β2-microglobulin and incubated with specified dilutions of normal human serum (NHS). After a total 4.5 hours of incubation at 37°C, supernatants were collected and tested for LDH activity to determine percent lysis.