Figure 3

Cell cycle distribution for breast cancer cells subjected to glutamine-mediated synchronization. Data are depicted as representative means for three independent experiments. Bcap37 cells (Figure 3a) and MCF7 cells (Figure 3b) were grown to confluency and either maintained in media with glutamine (Unsynchronized) or without glutamine for 48 hours (Gln Deprived), or without glutamine for 48 hours but then followed by 8 hours with glutamine (Gln Restored). All cells were harvested and treated with 0.5 mg/ml propidium iodide to determine cell cycle distribution by FACS analyses.