Figure 4

Expression of CD59 and CD55 in breast cancer cells subjected to glutamine-mediated synchronization. Data is depicted as representative means for three independent experiments. Bcap37 cells (Figure 4a) and MCF7 cells (Figure 4b) were grown to confluency and either maintained with glutamine (Unsynchronized) or in the same media without glutamine for 48 hours (Glutamine Deprived), or in the same media without glutamine for 48 hours followed by 8 hours with glutamine (Glutamine Restored). In all experimental groups for Bcap37 and MCF7 cells, CD59 was quantified using FITC-conjugated anti-CD59 mouse monoclonal antibody and CD55 was quantified using FITC-conjugated anti-CD55 mouse monoclonal antibody.