Figure 2

Changes in the size distribution and DNA content of cells during the cell cycle. Three independent experiments are presented (A-B, C-D, E-F). Each line is separated in time by one hour in graphs A-D, and by 1.33 hours in graphs E-F. (In graphs A and B the first two lines are separated by fifteen minutes with subsequent lines separated by one hour.) Newborn cells at the top are from the last fraction collected. Each subsequent line represents cells that have grown for one hour longer than the previous line (or 1.33 hours in graphs E and F.) The size distributions A, C, and E correspond with the respective DNA distributions B, D, and F. Exponential size and DNA distributions are shown at the top of panels E and F. The results shown in A-D are for 10% CCS while the panels E and F used 5% CCS.