Figure 5

Human 14-3-3γ suppresses appearance of mitotic cells in Drosophila eye imaginal discs. Eye imaginal discs from third instar larva from either control (A) or transgenic (B) flies were collected and stained with an anti-Phospho-Histone H3 (PH3) antibody which detects mitotic cells. GFP expression due to the presence of UAS-nlsGFP is shown in green. Phospho-Histone H3 staining is shown in red. Merged images are shown at the right. In photomicrograph "b" a white arrow points to a typical mitotic cell and in "b" and "e" white rectangular boxes mark the morphogenetic furrow and the second mitotic wave. In photomicrograph "c" the position the letter A marks the anterior portion of the eye disc and the letter P marks the posterior region. In "e" panel, SMW refers to second mitotic wave. The white arrows with the letters M ("f" and "g") depict mitotic cells. The letter P in photomicrograph "h" marks the posterior compartment of the eye disc. The white scales at the bottom right corners in "g" and "h" panels show 20 μm.