Figure 3

Cell cycle-dependent changes in cell morphology. Yeast cells were synchronized by nocodazole, released into the fresh rich media, and sampled every 20 min. The proportions of the cells showing the specific cell morphology at each time point calculated with CalMorph were plotted. Orange boxes on each figure indicate the approximate period of the cell cycle stage. A. Time-dependent changes in bud status. C119, C120, C121, and C122 indicate the proportion of unbudded cells, budded cells with a small bud, budded cells with a medium bud, and budded cells with a large bud, respectively. The schematic illustrations of the cell shape (orange) are depicted at the position on which the peak of each parameter value was observed. B. Time-dependent changes in nuclear status. D210, D211, D212, and D213 indicate the proportion of unbudded cells with one nucleus, budded cells with one nucleus in the mother, budded cells with one nucleus at the mother/bud neck, and budded cells with two nuclei (one each in the mother and bud), respectively. The schematic illustrations of the cell (orange) with a nucleus or nuclei (blue dots) are depicted at the position on which the peak of each parameter value was observed. C. Time-dependent changes in the actin status. A105, A106, A107, A108, A109, and A110 indicate the proportion of unbudded cells with dispersed actin patches, unbudded cells with localized actin patches, budded cells with localized actin patches at the bud tip, budded cells with dispersed actin patches in the bud, budded cells with dispersed actin patches in whole cell, and budded cells with localized actin patches at the mother/bud neck, respectively. Schematic illustrations of the cell (orange) with the actin patches (red dots) in the graph represent the peak position of the corresponding parameter value.