Figure 1
Strategy for in vivo imaging of p16INK4a gene expression. A large genomic DNA segment (195.4 kb) of human chromosome that contains the entire INK4a/ARF gene locus and surrounding sequences was engineered to express luciferase-tagged p16Ink4a. FISH technique reveals that the transgenic mice line (p16-luc) contanins a single copy of the human chromosome segment. The arrow shows the transgene. The p16-luc mouse was anesthetized and subjected to in vivo bioluminescence imaging after injection of luciferin.