Figure 5

Ectopic expression of human eIF3f in A549 cells. A) WB analysis of eIF3f expression in transfected pMSG (control) and pFH11S (ectopic expression) A549 cultures. Tubulin WB is shown to document equal protein loading. B) Difference in cell growth in control and overexpressed eIF3f cultures after 24 and 48 hours of transfection. Timepoint cero represents cells before transfection. Cells were stained with acridine orange and ethidium bromide to visualize cell morphology and death by necrosis or apoptosis. Final magnification: 40×. C) Percent of apoptosis in control (water and pMSG) and overexpressed eIF3f (pFH11S) transfected cells, estimated from cell counts in microscopic fields similar to those shown in panel B. Data expressed as mean ± SE; * p < 0.01 with respect to control (water or pMSG), n ≥ 3.