Coupling histone homeostasis to centromere integrity via the ubiquitin-proteasome system

Cell Division

Table 1 Fission yeast F-box proteins

			homologue		SCF/
F-box protein	motifs	recognition protein	yeast	human	*non-SCF	References
Pop1	WD40	Cig2, Cdc18, Rum1	Cdc4	Fbw7	SCF	[30, 34, 46]
Pop2	WD40	Cig2, Cdc18, Rum1	Cdc4	Fbw7	SCF	[30, 34, 46]
Pof1	WD40	Zip1	Met30	βTrCP	SCF	[47]
Pof2	LRR		Grr1	?	SCF	[25]
Pof3	LRR, TPR	Mcl1, Ams2	Dia2	?	SCF	[25, 28, 48]
Pof4	none		Ela1	ElonginA	SCF	[25]
Pof5	none		YDR360c	?	SCF	[25]
Pof6	CAAX		Rcy1	?	non-SCF	[49, 50]
Pof7	none		Hrt3	?	SCF	[25]
Pof8	none		Ufo1	?	non-SCF	[25, 51]
Pof9	none		YBR280c	?	SCF	[51]
Pof10	WD40		YML088w	?	SCF	[52]
Pof11	WD40		?	βTrCP	SCF	[51]?
Pof12	none		?	?	non-SCF	[25, 51]
Pof13	none		?	?	non-SCF	[25, 51]
Pof14	none	Erg9	?	?	SCF	[26]
Fbh1	DNA helicase	Atf1	?	Fbh1	SCF	[53–58]
SPAPB1A10.14	none		?	?	SCF	[51]

*Assignment of some F-box proteins (Pof6, Pof8, Pof12 and Pof13) as 'non-SCF' is based upon the fact that these F-box proteins, albeit binding Skp1, do not have the conserved proline residue within the F-box motif that is critical for direct interaction between F-box and Cullin-1 [59]. In fact, binding experiments showed this is the case [51]. The only exception is Pof9, which does not contain the proline residue, but it acts as a component of SCF.

ISSN: 1747-1028