Figure 5
From: The WW-HECT protein Smurf2 interacts with the Docking Protein NEDD9/HEF1 for Aurora A activation
Depletion of Smurf2 inhibits Aurora A activation at the centrosome. HeLa cells were transfected with control non-specific dsRNAs (siNS) or siRNA against Smurf2. At 40 h post-transfection, cells were fixed, stained with DAPI and pan-Aurora A or Thr288 phosphorylation-specific (pT288) Aurora A antibody, and subjected to immunofluorescence microscopy. Smurf2-depleted cells exhibit a marked reduction in Thr288 phosphorylated (active) Aurora A at the spindle poles.