Figure 4
From: GSK3 regulates the expressions of human and mouse c-Myb via different mechanisms
Effects of GSK3 on human c-Myb degradation. (A) Effects of GSK3 inhibitors on the stability of human c-Myb. Human c-Myb was transiently expressed with or without human Fbw7 in HeLa cells. The cells were treated with a GSK3 inhibitor, type II or SB216763, or DMSO for 24 h. The cells were then further treated with cycloheximide (CHX) at the indicated times of chase incubation and harvested. (B) Effects of depletion of GSK3β on the stability of human c-Myb protein. K562 cells were transfected with an siRNA for GSK3β or a control siRNA. At 48 h after transfection, the CHX assay was performed.