Figure 5
From: Self-organization of intracellular gradients during mitosis
Ran-GTP gradient. Local production of Ran-GTP by chromatin bound RCC1 produces a series of subordinate Ran-GTP dependent gradients that organize development of the mitotic spindle around chromatin. (A), Localized Ran-GTP production by RCC1 releases spindle assembly factors (SAF) from Importin beta around chromatin where they nucleate microtubules. (B), Diagram of Ran-GTP, SAF, Ran-GTP-Importin beta, and Ran-GTP-Importin beta-RanBP1 diffusion gradients that convey positional information to components of the developing mitotic spindle.