Gcn5 promoter occupancy is kept at an equilibrium in WT cells, but increases over time in hda1 cells. (A) Western analyses of Gcn5-HA expression in gcn5 Δ and gcn5 Δ hda1 Δ cells following a 5 hour 4% galactose-induction. Antibodies against GAPDH were used as a load control. (B) A galactose-induction time-course was performed in gcn5 Δ and gcn5 Δ hda1 Δ cells expressing GAL
-GCN5-HA. Protein samples were removed at the times shown for Western analyses with antibodies against HA and GAPDH. (C) From the time-course described above, samples were also removed for ChIP. Recovered DNA was used as a template in "end point" PCR reactions. S, sample with antibody; C, control without antibody; I, 10% lysate input. (D) The gel in (C) was scanned, analyzed using ImageJ and plotted.