From: Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles
 | Drosophila syncytial embryo (cycle 12) 10,11 | Drosophila S2 cells |
---|---|---|
Duration: | Â | Â |
NEB-telophase | 5-6 min | 40-50 min |
Pre-anaphase B steady state | 1-2 min | 30-40 min |
Pre-anaphase B spindle length | 10 μm | 8.6 μm |
Anaphase B spindle length change | 5 μm | 5 μm |
MT flux rates: | Â | Â |
Pre-anaphase B | 0.053 ± 0.013 μm/s | 0.05 ± 0.006 μm/s |
Anaphase B | 0.008 ± 0.019 μm/s | 0.03 ± 0.007 μm/s |
Anaphase B rate | 0.08 ± 0.015 μm/s | 0.017 μm/s |
Chromosome segregation rate | 0.11 ± 0.019 μm/s | 0.0098 ± 0.0047 μm/s |
MT plus end distribution at anaphase B | Redistribution to the equator | Partial redistribution to the equator |
MT turnover at pole: | Â | Â |
Pre-anaphase B | 81.0 ± 14.3% | 113.1 ± 12.3% |
Anaphase B | 45.8 ± 11.2% | 71.8 ± 21.2% |