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Table 1 Summary of comparative data

From: Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles

 

Drosophila syncytial embryo (cycle 12) 10,11

Drosophila S2 cells

Duration:

  

NEB-telophase

5-6 min

40-50 min

Pre-anaphase B steady

state

1-2 min

30-40 min

Pre-anaphase B spindle

length

10 μm

8.6 μm

Anaphase B spindle length

change

5 μm

5 μm

MT flux rates:

  

Pre-anaphase B

0.053 ± 0.013 μm/s

0.05 ± 0.006 μm/s

Anaphase B

0.008 ± 0.019 μm/s

0.03 ± 0.007 μm/s

Anaphase B rate

0.08 ± 0.015 μm/s

0.017 μm/s

Chromosome segregation

rate

0.11 ± 0.019 μm/s

0.0098 ± 0.0047 μm/s

MT plus end distribution

at anaphase B

Redistribution to the equator

Partial redistribution to the equator

MT turnover at pole:

  

Pre-anaphase B

81.0 ± 14.3%

113.1 ± 12.3%

Anaphase B

45.8 ± 11.2%

71.8 ± 21.2%