Figure 1

hos2 ∆ and hos2-Y321H mutants are hyper-sensitive to LatA treatment. (A) Ten-fold serial dilutions of logarithmically growing cells of the indicated genotype were plated onto YES plates containing 0.5 μM LatA or DMSO (solvent control) at 30°C for 3 d. (B) Cells of the indicated genotype were grown to mid-log phase at 30°C and then treated with 0.5 μM LatA for 5 h before being fixed and stained with DAPI (nuclei) and aniline blue (cell wall/septa). Bar, 10 μm. (C) Quantitation of phenotypes of cells treated as in B. Between 200 and 500 cells were counted for each genotypic class.