Figure 4

Model for HAUS- and γ-TuRC-mediated MT generation within the mitotic spindle (adapted from Uehara et al. [63]). The 8-subunit HAUS complex, which resides at the centrosome in interphase, recruits γ-TuRCs from centrosomes to spindle MTs in mitosis, where γ-TuRC nucleates additional MTs. HAUS8/HICE1 directly binds to MTs and to HAUS6/FAM29A, which in turn binds the γ-TuRC via NEDD1. HAUS complex and γ-TuRC are shown in yellow and blue, respectively. The components of both complexes are indicated in the magnified view. Spindle-templated MT amplification mediated through interaction of the HAUS complex and the γ-TuRC was demonstrated to be critical for maintenance of centrosome and spindle integrity, for the generation of tension between sister kinetochores and for the completion of cytokinesis.