Table 2 Plasmids used in this study
From: APC/C-Cdh1-dependent anaphase and telophase progression during mitotic slippage
Name (Alias) | Description (Source) |
|---|---|
pSCU134 (p416GAL1) | GAL1 URA3 CEN [64] |
pSCU145 (pRS414) | TRP1 CEN [65] |
pSCU563 (pLacOx256-LEU2) | lacOx256 LEU2 integrative [26] |
pSCU564 (pGFP12-LacI12-NLS) | CUP1pro-GFP12-LacI12-NLS HIS3 integrative [26] |
pSCU683 (ptetR-GFP) | NLS-tetR-GFP LEU2 integrative [66] |
pSCU710 (ptetO2x112) | tetO2x112 URA3 integrative [66] |
pSCU740 (pNOP1-GFP) | NOP1-HA3GFP URA3 CEN (this study) |
pSCU784 (pPDS1-HA3) | PDS1-HA3 URA3 integrative [31] |
pSCU802 (pGAL1-CDC14) | GAL-CDC14-His6 URA3 CEN [67] |
pSCU816 (pGAL-SCC1-RRDD) | GAL-SCC1-R180D/R268D-HA3 LEU2 integrative [40] |
pSCU878 (pGAL-CDH1) | GAL-CDH1-GFP TRP1 CEN [68] |
pSCU896 (pGAL1-BFA1) | GAL1-BFA1 URA3 CEN [69] |
pSCU973 (pMAD2-GFP) | MAD2-GFP URA3 CEN [70] |
pSCU985 (YIp22-MET3-CDC20) | MET3-CDC20 TRP1 integrative (F. Uhlmann) |
pSCU1212 (p416GAL1-pds1-db) | GAL1-PDS1 with mutated D-box URA3 CEN (this study) |
pSCU1214 (p416GAL1-pds1-dkb) | GAL1-PDS1 with mutated D/KEN-box URA3 CEN (this study) |
pSCU1550 (pGAL1-MAD2) | GAL1-MAD2-His6HAZZ 2 μ URA3 [71] |
pSCU1575 (pGAL1-CDH1) | GAL1-CDH1-TAP 2 μ TRP1 (this study) |
pSCU1576 (pGAL1-CDC14) | GAL1-CDC14-His6HAZZ 2 μ TRP1 (this study) |
pSCU1701 (pMTW1-RFP) | MTW1-DsRed.T4 CEN LEU2 (this study) |