Characterization of NIH3T3 cells over-expressing hPiT1. A) qRT-PCR analysis of exogenously expressed hPiT1 in NIH3T3-LPiT1SN (LPiT1SN) and NIH3T3-LXSN (LXSN) cells. Each column represents cell lysates from three wells and triplicate qRT-PCR analyses of each cell lysate. The hPiT1 mRNA levels are standardized to the endogenous B2M mRNA levels. Data are means ± SD.* indicates statistically significantly different from NIH3T3-LXSN (control) cells. B) RBD-binding assay. Analyses of binding of His-tagged FeLV-B RBD and A-MLV RBD to NIH3T3-LXSN (LXSN) and NIH3T3-LPiT1SN (LPiT1SN) cells were performed as described in the legend to Figure 3. Incubations with His-tagged FeLV-B RBD containing supernatant (dashed line) or standard growth medium (solid line) are shown. The x-axis shows PE intensity measured in the FL2-H channel.