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Table 1 Quantitative analysis of lethal and mitotic phenotypes of lmg mutants

From: lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2

Genotype Lethal phase Preparations per fields Mitotic index M:A ratio Polyploid % Apoptotic index
A       
w 11118 Viable 7/183 1.6 ± 0.2 2.9 ± 0.3 0 0.5 ± 0.1
lmg 03424 Pharate adult P15(i) 6/135 4.1 ± 0.8 4.5 ± 0.7 19.6 ± 5.6 1.4 ± 0.3
lmg J023 Pupal stage P5(i) 7/167 4.4 ± 0.9 6.3 ± 0.1 29.1 ± 7.4 1.2 ± 0.2
lmg EY11317 Prepupal stage P4(ii) 6/170 5.8 ± 0.7 6.2 ± 0.4 26.6 ± 3.1 1.5 ± 0.4
lmg 138 Prepupal stage P4(i) 10/194 5.9 ± 0.5 - 50.7 ± 7.9 1.8 ± 0.5
lmg 138 ;lmgA- pUAST/daGAL4 Viable 6/180 1.5 ± 0.2 3 ± 0.6 0 0.5 ± 0.1
lmg 138 ;lmgA + B- pUAST/daGAL4 Viable 4/100 1.4 ± 0.2 3.1 ± 0.3 0 0.6 ± 0.2
lmg 138 ;lmgB- pUAST/daGAL4 Prepupal stage P4(i) 5/100 4.9 ± 0.9 - 43.7 ± 6.2 1.7 ± 0.2
B       
mr 2 Female sterile 6/174 1.4 ± 0.1 3.3 ± 0.4 0 1.2 ± 0.3
lmg 03424 , mr 2 Prepupal stage P4(ii) 6/106 4.5 ± 0.7 4.4 ± 0.3 31.8 ± 3.9 1.3 ± 0.4
C       
vihar S110501 Pharate adult P15(i) 7/196 2.1 ± 0.3 3.7 ± 0.6 4.2 ± 0.4 0.9 ± 0.3
lmg 03424 , vihar S110501 Bubble prepupa 6/116 6.1 ± 0.6 3.9 ± 0.7 44.3 ± 8.4 1.8 ± 0.3
  stage P3      
mr 2 ; vihar S110501 Early L3 larvae n.d. n.d. n.d. n.d. n.d.
mr 2 ; vihar KG02013 Early L3 larvae n.d. n.d. n.d. n.d. n.d.
  1. The number of preparations and the total number of optical fields analyzed are given. Mitotic index is given as the average number of mitotic cells in an optical field (± sd). Polyploid% represents the percentage of mitotic cells (± sd) with higher than two sets of chromosomes. Apoptotic index is given as the mean number of apoptotic cells in a microscope field (± sd).