Quantitative expression of KBTBD8 on mouse tissues and cell lines. A: Western Blot analysis on mouse cell lines using a Kbtbd8 specific antibody revealed a high protein expression of KBTBD8 in differentiated ESCs and maGSCs as well as in NIH3T3 cells and a low one in ESCs. However, in maGSCs no KBTBD8 protein could be detected. α-Tubulin served as loading control. B: qRT-PCR results support the Western Blot results. C: Western Blot analysis on different mouse organs revealed an ubiquitous expression in all tested organs. β-Actin served as loading control. D: qRT-PCR results support the Western Blot results. Abbreviations: ESC: Embryonic Stem Cell; ESCdiff.: differentiated Embryonic Stem Cells; maGSC: multipotent adult Germline Stem Cell; maGSCdiff.: differentiated multipotent adult Germline Stem Cell.