Figure 3

Altered KBTBD8 expression results in the dislocation of Golgi structures. To test the specificity of the KBTBD8 antibody and to see, if there is an effect on the Golgi apparatus, NIH3T3 cells where transfected with siRNA against Kbtbd8. A: qRT-PCR results followed by statistical analysis using t-test revealed the significant downregulation of Kbtbd8 mRNA in two biological replicates. B: Western Blot results support the downregulation of KBTBD8 even on the protein level. C: Immunocytochemistry using GM130 and KBTBD8 specific antibodies revealed an altered localisation of the Golgi matrix protein GM130. In cells, were KBTBD8 expression is downregulated or absent upon siRNA transfections, the localization of the GM130 signal becomes rearranged (white arrows).