Nucleolar localization of CBP4a is Ca2+−
dependent. (A) Immunolocalization of CBP4a in BAPTA-treated cells. Immunolocalization of α-tubulin was used to verify cellular integrity and DAPI was used to identify the nucleus. A merged image shows the overlay of CBP4a (red), α-tubulin (green), and DAPI (blue). (B) Immunolocalization of CBP4a or NumA1 in the nucleus of either untreated or BAPTA-treated cells. Arrows denote nucleoli. Scale bar represents 2 μm. At least four independent replicates were performed, all of which showed the same result.