Skip to main content

Table 1 Yeast strains used in this study

From: Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast

Strain

Genotype

Source

RSY335

MAT a /MATα cyh2 r -z ho::LYS2 leu2::hisG lys2 trp1::hisG ura3

[63]

RSY562

ama1::KANMX4

[10]

RSY750

AMA1-3HA CDC20-18MYC::URA3

This study

RSY776

MAT a cdh1::LEU2

This study

RSY777

cdh1::LEU2

This study

RSY809

cdc20-1

[32]

RSY954

cdc16-1

This study

RSY1248

MAT a CDC16::TAP mnd2::KANMX4

This study

RSY1337

MATα ade2 ade6 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 CDC27-9myc::LEU2

This study

RSY1381

MAT a CDC16::TAP::KAN/CDC16 mnd2::KANMX4 cdh1::LEU2

This study

RSY1748

MAT a CDC16::TAP/CDC16 mnd2::KANMX4 cdh1::LEU2 doc1:: TRP1

This study

KCY328

CDC27-9myc::LEU2

This study

KCY419

MATα mnd2::KANMX4

This study

KCY456

MAT a CDC16::TAP

This study

  1. *All strains, except RSY1337 are isogenic to RSY335. All strains are diploids and all alleles are homozygous unless indicated.