Table 1 List of S. cerevisiae strains used in this study
From: The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence
Strains (ORF name) | Genotype | Source |
|---|---|---|
TOF1-GFP (YNL273W) | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP::His3MX6 | Invitrogen™ |
MRC1-GFP (YCL061C) | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 | Invitrogen™ |
CSM3-GFP (YMR048W) | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-HIS3MX6 | Invitrogen™ |
RAD53-GFP (YPL153C) | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad53-GFP-HIS3MX6 | Invitrogen™ |
TOF1-GFP; csm3Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP-HIS3MX6 csm3Δ::KanMX | This study |
TOF1-GFP; mrc1Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP-HIS3MX6 mrc1Δ::KanMX | This study |
CSM3-GFP; tof1Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-His3MX6 tof1Δ::KanMX | This study |
CSM3-GFP; mrc1Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-HIS3MX6 mrc1Δ::KanMX | This study |
MRC1-GFP; csm3Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 csm3Δ::KanMX | This study |
MRC1-GFP; tof1Δ | MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 tof1Δ::KanMX | This study |
HTB2-mCherry | SLJ3517 (MATα, htb2::HTB2-mCherry-HYGMX) | Sue L. Jaspersen |