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Table 1 List of S. cerevisiae strains used in this study

From: The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence

Strains (ORF name) Genotype Source
TOF1-GFP (YNL273W) MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP::His3MX6 Invitrogen™
MRC1-GFP (YCL061C) MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 Invitrogen™
CSM3-GFP (YMR048W) MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-HIS3MX6 Invitrogen™
RAD53-GFP (YPL153C) MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 rad53-GFP-HIS3MX6 Invitrogen™
TOF1-GFP; csm3Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP-HIS3MX6 csm3Δ::KanMX This study
TOF1-GFP; mrc1Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 tof1-GFP-HIS3MX6 mrc1Δ::KanMX This study
CSM3-GFP; tof1Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-His3MX6 tof1Δ::KanMX This study
CSM3-GFP; mrc1Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 csm3-GFP-HIS3MX6 mrc1Δ::KanMX This study
MRC1-GFP; csm3Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 csm3Δ::KanMX This study
MRC1-GFP; tof1Δ MAT a his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 mrc1-GFP-HIS3MX6 tof1Δ::KanMX This study
HTB2-mCherry SLJ3517 (MATα, htb2::HTB2-mCherry-HYGMX) Sue L. Jaspersen