Fig. 2

The DNA binding and repression domains of the TBX3 protein are required for the repression of p21. a Schematic representation of the human wild-type (WT) TBX3 protein (WT-TBX3), TBX3 N terminal (TBX3 N-term) and TBX3 DNA-binding domain mutant (DBM) expression constructs. “A” and “R” denotes activation and repression domains respectively. b ATDC5 and c SW1353 cells were co-transfected with a p21 promoter luciferase reporter construct together with pCMV empty, WT TBX3, TBX3 DBM or TBX3 N-term expression constructs. The plasmid pRL-TK containing the Renilla luciferase reporter gene was also introduced to normalize transfection efficiency. Fold repression was obtained by comparing the results to that of the empty pCMV vector transfection. b, c The values indicate the mean of three independent experiments ± SEM (*p < 0.05; **p < 0.01, ***p < 0.001). Lower panels western blotting shows expression of HA-tagged WT TBX3, TBX3 N-term and TBX3 DBM proteins using a HA antibody