Fig. 1

Rpl22 is a nonessential protein required for hyphal growth. a Wild type (RSY333), rpl22AΔ (RSY1479), rpl22BΔ (RSY1446) and rpl22Δ double mutant (RSY1483) cultures were incubated at the indicated temperatures for 24 h in rich (YPD) liquid culture then spotted onto rich agar plates and incubated at 23° (left panel). The same cultures were grown in rich medium at 23° for 5 days, then a dilution series was spotted on rich agar and incubated at 23° for 2 days. b The strains described in a were streaked on YPD agar plates and incubated at 19 °C for 4 days (left panel), then 30 °C for 2 days (right panel). c Wild type (RSY333), rpl22AΔ (RSY1479) and rpl22BΔ (RSY1446) cultures were grown to mid-log and total RNA preparations were subjected to qRT-PCR analysis. RPL22A and RPL22B mRNA levels were normalized to NUP85 in wild type then analyzed in each single mutant. ENO1 mRNA levels were quantified as a RNA concentration control. The results are referenced to wild type levels ± SEM (n = 3). d Haploid strains described in a were streaked on rich growth medium for 2 days (left panel), then subjected to a stream of water, the re-incubated for 24 h (right panel). e SK1 derived diploid wild type (RSY877) and rpl22Δ (RSY1823) double mutant cells were assayed for pseudohyphal growth by microscopy (×60 final magnification)