Fig. 6

Rpl22 is required for normal polysome profiles under conditions of high translational output. a Logarithmically growing wild type (RSY335), b rpl22AΔ (RSY1559), c rpl22BΔ (RSY1560) and d rpl22Δ double mutant (RSY1561) cells in rich dextrose medium (YPD) were subjected to polysome profile analysis through sucrose density gradient centrifugation. The arrows indicate the 40S, 60S, 80S monosome and halfmer positions. e Overlaid polysome profiles for wild type and rpl22∆ strains taken 9 h following transfer to SPM medium. f The 25S:18S rRNA ratios were quantified by Northern blot analysis for wild type and the rpl22∆ double mutant in log phase cells grown in rich dextrose (YPD) medium. The cumulative differences between the 25S:18S ratios in wild type and rpl22∆ double mutant strains were significantly different (p = 0.02) in YPD but not SPM