Fig. 6

Effect of Eg5 inhibition on the distribution of cortical granules and ovastacin in porcine oocytes. a Representative images of cortical granule localization in control and Eg5-inhibited oocytes. Oocytes were immnunostained with LCA-FITC to visualize the cortical granules. Scale bar, 40 μm. b The fluorescence intensity of cortical granules was measured in control and Eg5-inhibited oocytes. c Representative images of ovastacin localization in control and Eg5-inhibited oocytes. Ovastacin was immunostained with rabbit polyclonal anti-human ovastacin antibody and imaged by confocal microscopy. Scale bar, 40 μm. d The fluorescence intensity of ovastacin was measured in control and Eg5-inhibited oocytes. Data in b and d were presented as mean percentage (mean ± SEM) of at least three independent experiments. **p < 0.01