Fig. 4
From: IMP/GTP balance modulates cytoophidium assembly and IMPDH activity
Mutations on CBS domain prevent cytoophidium assembly. a Mutant cell lines were treated with 1Â mM ribavirin for 4Â h and probed with mouse monoclonal anti-IMPDH1 and rabbit polyclonal anti-IMPDH2 antibody to evidence the no-filament phenotype. The arrows in indicate the previously described intranuclear filaments. b Cell lines with endogenous mutations induced by CRISPR/Cas9 system on IMPDH2 gene. Sequences showing the genotype of each cell line