Fig. 3

Cell cycle profiles obtained with varying cellular densities. Asynchronous cells were plated into 8-well coverglass-bottom chambered slides at varying densities and allowed to adhere for 24 h. Hoechst 33342 was added to the imaging medium and allowed to reach binding saturation (~ 2 h). Images for Hoechst 33342 were then collected at distinct locations and histograms generated from images containing approximately 100, 200, 300, 400, 500 and 600 cells within a single field of view. The x-axis represents normalized integrated nuclear fluorescence for each cell imaged and the y-axis histogram counts