Fig. 5

Spindle assembly and chromosome alignment demand hypoacetylation of tubulin K40 during meiosis. a Typical images of MI control oocytes, Kif18a siRNA oocytes and Kif18a siRNA + tubulin K40R mRNA-injected oocytes. Green, α-tubulin; red, DNA. b The incidence of abnormal spindles and misaligned chromosomes in control oocytes, Kif18a siRNA oocytes and Kif18a siRNA + tubulin K40R mRNA-injected oocytes. Data are presented as mean percentage (± SEM) of at least three independent experiments. *p < 0.05; scale bars: 20 μm