Fig. 6

Interaction of 3,4,4′-TMS and related compounds with γ/γ- and α/β-tubulin dimers. a Predicted mode of interaction of 3,4,4′-TMS, 3,5,4′-TMS, combretastatin A4 and colchicine with γ/γ-tubulin dimers. The two γ-tubulin monomers are shown as ribbon and coloured gold and cyan, respectively. Both the GDP present in the structure and the docked ligands are shown as sticks and coloured by atom type: N, blue; O, red; P, orange; C, green, salmon, blue, yellow and grey in 3,4,4′-TMS, 3,5,4′-TMS, combretastatin A4, colchicine and GDP, respectively. b Predicted mode of interaction of 3,4,4′-TMS, 3,5,4′-TMS, combretastatin A4 and colchicine with α/β-tubulin dimers. The α- and β-tubulin monomers are shown as ribbon and coloured light and dark green, respectively. Both the GTP and GDP inherited from the template and bound to α- or β-tubulin monomers, respectively, and the docked ligands are shown as sticks and coloured as in a. c Close view of the interactions between 3,4,4′-TMS and γ-tubulin. 3,4,4′-TMS is coloured as in a. γ-tubulin residues having at least one atom within 4.0 Å from 3,4,4′-TMS are labelled (residues belonging to the two monomers are differentiated by the absence and presence of an apex, respectively), shown as sticks and coloured by atom-type: N, blue; O, red; C, orange and light blue for the γ-tubulin monomer on the left and on the right, respectively. Hydrogen bonds are indicated by dashed lines. d Close view of the interactions between 3,5,4′-TMS and γ-tubulin. 3,5,4′-TMS is coloured as in a. γ-tubulin residues having at least one atom within 4.0 Å from 3,5,4′-TMS are shown as in c. Hydrogen bonds are indicated by dashed lines