Fig. 3

Septin marks the presence of an ICB. M-cells were isolated and either re-plated on fibronectin-coated dishes (adhesion) or non-adhesive dishes (suspension) for the indicated time points. Representative immunofluorescence images illustrating the localization of septin-7 (green) and anillin (red) in the adhesion and suspension conditions in a BJ cells, c BJ-LT cells, and e BJ-LT-Ras cells. Nuclei were stained with DAPI (blue). Note that for BJ and BJ-LT-Ras two examples are given at the 42- and 3-h time points, respectively, illustrating different septin-7 staining. Under adherent conditions, all three cell types had divided after 3 h (here shown only for BJ cells; see Additional file 5: Figure S3). b, d, f Mean% ± SD of the number of cells without septin-7 along ICB at the indicated time points for non-adherent BJ, BJ-LT, and BJ-LT-Ras cells. Difference significance: BJ-LT-Ras 3 h vs. BJ 3 h or BJ-LT 3 h***