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Fig. 5 | Cell Division

Fig. 5

From: Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization

Fig. 5

PIP2 binds to PLK1 and reduces PLK1-mediated phosphorylation of MCAK. a A representative blot showing the binding of PLK1 to PIPs. The PIP strip was incubated with recombinant human PLK1. The binding of PLK1 to each PIP was then detected and visualized as described in “Methods” section. b PIP2 reduces the phosphorylation of MCAK by PLK1. The MCAK immunoprecipitated from cells was incubated with recombinant PLK1, and MCAK phosphorylation was verified by immunoblotting with anti-phospho-serine antibody as described in “Methods” section

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