Fig. 1

The expression of LHX9 was up-regulated in OS tissues and cell lines. a The relative expression of LHX9 in normal tissues (solid dots) and OS tumor tissues (solid squares) excised from patients was assessed by RT-qPCR. LHX9 mRNA levels were normalized to GAPDH mRNA (n = 3). b The correlation analysis between the expression of LHX9 and the survival of patients with OS. Blue line: low and medium LHX9 expression; red line: high LHX9 expression. c The relative expression of LHX9 in OS cell lines 143B, U2OS, MNNG-HOS and Saos-2 was measured by RT-qPCR. hFOB.19 was the control cell line. The mRNAs were normalized to GAPDH mRNA (n = 3). d The expression of LHX9 in OS cell lines 143B, U2OS, MNNG-HOS and Saos-2 was determined by Western blot. hFOB.19 was the control cell line, GAPDH was used as the loading control. The relative expression of LHX9 after normalization to GAPDH was shown in the right histogram. a–d The result was a representative of three independent experiments. a, c, d Error bars represented mean ± SD. p values were determined by one-way analysis of variance (ANOVA) followed by Tukey post hoc test (c, d), unpaired two-tailed Students’ t-test (a) or log-rank test (b). **p < 0.01, *p < 0.05