Fig. 4

LHX9 knockdown led to the down-regulation of BMP-4, β-catenin, COL1A1, MMP-1, Snail-1, Slug-1, Twist1 and MMP-9. a, b U2OS or Saos-2 cells were transfected with shNC or shLHX9, then they were cultured for 48 h. The expression of LHX9, BMP-4, β-catenin, COL1A1, MMP-1, Snail-1, Slug-1, Twist-1, MMP-9 (a) and FRS2, TGF-βR1 (b) was measured by Western blot. GAPDH was used as the loading control. The normalized expression of these proteins against GAPDH was shown in the right histograms. The result was a representative of three independent experiments. Error bars represented mean ± SD. p values were determined by unpaired two-tail Students’ t-test. **p < 0.01, *p < 0.05. NS not significant