Fig. 1

Cell cycle genes have distinct essentiality signatures in pluripotent and cancer cells. a Venn diagram showing the overlap of cell cycle gene sets examined in this study. b CRISPR score distributions of cell cycle genes in ESCs (red curve) and cancer cells (orange curve). CRISPR score per gene for cancer cells represents the average score across four transformed cell lines. P-value of Kruskal Wallis test is shown. c PCA plot demonstrating the separation of essentiality scores for cell cycle genes across different cell lines. d Fraction of checkpoint genes among all cell cycle genes as compared to their fraction among the top 100 genes contributing to PC1 separation