Fig. 4

Inhibit IGFBP5 promotes GBM cell proliferation. a, c CCK-8 assay were used to explore the U251 and G1 cell proliferation after transfected with siBP5 or si-NC for 24 h or 48 h. b, d Akt and pAkt were detected by Western blot analysis and β actin was used as an internal control, the corresponding semi-quantitative analysis of pAkt(Ser473)/Akt ration was based on optical density with ImageJ software. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001