Fig. 4

EZH2 inhibition aggravated cisplatin-induced apoptosis in NRK-52E cells. NRK- 52E cells were stimulated with 20 μM DZNep for 1 h, followed by 20 μM cisplatin (CP) stimulation for 24 h. Flow cytometry analysis was conducted (a). Western blots show the protein level of cleaved-caspase 3 (b), S6k, p ~ S6k (c), Akt and p ~ Akt (d). GAPDH was used to verify equivalent loading. Quantitative data are presented. Data are given as the mean ± SD of three independent experiments. *p < 0.05 versus vehicle- treated cells. #p < 0.05 versus cells treated with cisplatin alone