Fig. 2

Chl1p is not required for G2/M arrest after DNA damage by MMS. Wild-type (699) and mutant cells 699Dchl1 (chl1) and SL3 (rad24) were grown to exponential phase and arrested with 15 μg/ml nocodazole for 3 h at 30 °C for G2/M arrest. The arrested cells were treated with 0.15% MMS during last half an hour of nocodazole arrest and kept shaking. After treatment, MMS was inactivated by the addition of one volume of 10% sodium thiosulfate solution. Cells were washed with YEPD medium and released in fresh medium at 30 °C and aliquots were removed at regular intervals and stained with DAPI to score for the percentage of cells with divided nuclei. Data shown are average of values obtained from three independent experiments and error bars are standard deviations from the mean value. The filled symbols are given for cells treated with MMS, the empty symbols indicate the absence of MMS. Error bars are not shown for data points pertaining to minus MMS experiments to avoid cluttering