Fig. 2
From: ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs
Effects of ROMO1 knockdown on early porcine embryonic development. A Quantitative PCR was conducted to confirm ROMO1 knockdown at the blastocyst stage. 18 s was selected as reference gene. B Western blotting was conducted to confirm ROMO1 knockdown at the blastocyst stage. C The morphology of the d-7 embryos in dsROMO1 and dsControl groups. Scale bars, 100 μm. D The embryo development rate from 2-cell to blastocyst stages in dsROMO1 and dsControl groups. E Blastocyst diameter of dsROMO1 (n = 40) and dsControl (n = 40) embryos. F Total cell number of dsROMO1 (n = 60) and dsControl (n = 60) embryos. **P < 0.01, ***P < 0.001 and ****P < 0.0001 indicate significant differences between treatment groups