Table 1 Summary of PCR primer
From: ROMO1 is required for mitochondrial metabolism during preimplantation embryo development in pigs
Gene | Accession | Primer sequence, 5ʹ-3ʹ | Product length (bp) | Used for |
|---|---|---|---|---|
ROMO1 | XM_001924066 | F: cgttcggtgtgagacgtaga | 189 | dsRNA |
R: cgcattccgatcctgagaca | ||||
qROMO1 | XM_001924066 | F: tcggcaccttttcctgtctc | 186 | qRT-PCR |
R: acatgggctgggactgattg | ||||
Mch-ROMO1 | XM_001924066 | F: agatctcgagagatgcctgtggccgtg | 267 | mRNA |
R: atctagagcggccgcttagcatcggatgcccatc | ||||
eGFP-ROMO1 | XM_001924066 | F: aagtccggactcgagatgcctgtggccgtg | 267 | mRNA |
R: cttgagctcgagttagcatcggatgcccatc | ||||
18S | NR_046261 | F: cgcggttctattttgttggt | 219 | qRT-PCR |
R: agtcggcatcgtttatggtc |