Table 1 Summary of experimental evidence implicating the activation of cell cycle regulators on the yeast SPB or metazoan centrosome in regulating cell cycle transitions
From: An updated view on the centrosome as a cell cycle regulator
Protein/protein complex | Experimental evidence |
|---|---|
Aurora A | Centrosomal localisation observed in human cells [71] Experimental evidence supporting a role in G2/M transition [71] Localised activation at the centrosome suggested from RNAi experiments in C. elegans [76] |
Cdc25 | Centrosomal localisation in interphase observed in human cells [70] Localised activation at the centrosome in triggering G2/M transition suggested in human cells from siRNA experiments [70, 152] |
Cdk 1-Cyclin B | SPB localisation observed in fission yeast cells [60, 123] Experimental evidence demonstrating the localised activation at the SPB promotes G2/M transition in fission yeast [122, 123] Centrosomal localisation in G2 observed in human cells [56, 58] Activation of the complex at the centrosome was observed in G2 before spreading into the nucleus before mitosis in human cells [61] |
Cdk 2- Cyclin E | Centrosomal localisation in interphase observed in Xenopus [48] Experimental demonstration of localised activation at the centrosome promotes G1/S transition in human cells [49,50,51] |
Chk1 | Centrosomal localisation in interphase observed in human cells [70, 99] Centrosomal localisation is impaired when PCM genes are mutated/disrupted suggesting a role in G2/M cell cycle arrest in human cells [92, 97, 98] Experimental evidence demonstrating localised activation at the centrosome results in cell cycle arrest in human cells [99] |
Chk2 | SPB localisation observed in fission yeast [144] Experimental evidence demonstrating localised activation at the SPB supports mitotic entry in fission yeast [144] Localisation of protein in the centrosome observed in human cells [153,154,155] |
PIDDosome | Centrosomal localisation observed in human cells [104,105,106] Localised activation at the centrosome is required for the stabilisation of the p53 [104, 105] |
Plk1 | SPB localisation observed in yeast cells [122, 128, 156] Localised activation at the SPB induced G2/M transition in fission yeast cells [122] The cellular kinase activity of Plk1 influenced localized recruitment of PP1 on the yeast SPB [128, 129] Centrosomal localisation observed in human cells [75, 155] Localised activation regulating G2/M transition from the centrosome suggested from drug inhibition and RNAi experiments in human cells [73,74,75] Localised activation at the centrosome promoting mitotic entry was proposed from RNAi experiments in C. elegans [76] |
Wee1 | SPB localisation in interphase observed in yeast cells, cell cycle regulatory role proposed based on changes in distribution during cell cycle progression [157, 158] Centrosomal localisation in interphase observed in human cells [70, 159] |