Fig. 2

dGLYAT is necessary for cell polarity disruption-induced EMT-like phenotype. Fluorescent micrographs of third instar larval wing discs are shown (a-d). Compared with the ptc > GFP control (a), ptc > scrib-IR induced cell migration and reduced E-cadherin expression in the A/P boundary (b, arrow), which were suppressed by expressing dGLYAT RNAi (d), but not LacZ RNAi (c). Detailed genotypes: a ptc-Gal4 UAS-GFP/+, b ptc-Gal4 UAS-GFP UAS-scrib-IR/+, c ptc-Gal4 UAS-GFP UAS-scrib-IR/+; UAS-LacZ-IR/+, d ptc-Gal4 UAS-GFP UAS-scrib-IR/+; UAS-dGLYAT-IR/+